Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.
نویسندگان
چکیده
منابع مشابه
Production and Regeneration of Protoplasts from Orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp
The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10 protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regenerati...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2010
ISSN: 1516-8913
DOI: 10.1590/s1516-89132010000100019